（一）个人简介
杜志游（1978）
男，教授，博士，博士生导师，博士后合作导师
剑桥大学博士后，马里兰大学研究助理
特殊人才计划：浙江省“万人计划”科技创新领军
学科方向：微生物学，病毒学
研究方向：分子病毒学、RNA结构生物学
（二）工作经历
2020.01-至今 教授，浙江理工大学，生命科学与医药学院
2015.10-2017.09 研究助理，马里兰大学，细胞生物学与分子遗传学系
2011.10-2015.09 副教授，浙江理工大学，生命科学学院
2009.10-2011.09 博士后，剑桥大学，‘玛丽·居里’研究学者
2007.04-2009.09 副研究员，浙江理工大学，生命科学学院
2001.09-2006.09 理学博士，浙江大学，微生物学专业
1997.09-2001.07 理学学士，温州师范学院，生物科学专业
（三）奖励荣誉
1) 浙江省“万人计划”科技创新领军人才（2026）
2) 浙江省151人才培养工程 （2010）
3) 浙江理工大学首批521中青年拔尖人才（2013）
4) 浙江省政府应急专家
5) 浙江理工大学“三育人”荣誉称号
6) 浙江理工大学“十佳班主任”荣誉称号
7) 浙江理工大学“优秀班主任”荣誉称号
（四）项目情况
1. RNA沉默通路在CMV卫星RNA引发番茄致死性坏死中的作用机制，国家自然科学基金(3257010514),2026.01-2029.12,直接经费50万（主持）
2. NMD靶向降解CMV基因组RNA3的分子机理及其生物学意义，国家自然科学基金（32070154），2021.01-2024.12，直接经费58万（主持）
3. 卫星RNA选择性抑制CMV RNA1和RNA2复制的结构基础及其作用机制, 国家自然科学基金（31870144），2019.01-2022.12，直接经费59万（主持）
4. 植物液泡脂质调控CMV复制的分子机制，国家自然科学基金（31600125），2017.01-2019.12，直接经费22万（1st参加）
5. CMV复制酶组分1a蛋白调控亚基因组RNA4A合成的分子机制，国家自然科学基金（31470007）， 2015.01-2016.12，30万（主持）
6. 卫星RNA和协生病毒与CMV的互作对CMV诱导拟南芥抗蚜虫反应的影响及其途径,国家自然科学基金（31170141），2012.01-2015.12，64万（主持）
7. Subversion of Insect Resistance: A novel Role for a Plant Viral Silencing Suppressor. European FP7-Marie Curie International Incoming Fellowship （欧盟第七框架-玛丽居里国际来访学者项目）(PIIF-GA-2009-236443), 2009.10-2011.09，约175万（主持）
8. CMV 2b蛋白致病关键氨基酸在RNA沉默通路中的作用方式和靶位,国家自然科学基金（30800043），2009.01-2011.12，20万（主持）
9. 外源AGO1蛋白特定片段对CMV 2b蛋白抗RNA沉默活性的干扰研究,浙江省自然科学基金（Y3080315），2009.01-2011.12，8万（主持）
10. 蜂源性降血压功能肽高效表达基因的筛选及优化,国家高技术发展研究计划（863计划）, 2008.01-2010.12，50万（主持）
（五）成果（论文/专利）
---第一或通讯作者论文（*通讯作者）
1. Kagimbo, J.A., Du, Z*. Global advances in research on plant resistance genes: an updated compilation and bibliometric analysis. Phytopathol Res 8, 13 (2026).
2. Zhao D, Ahmed MR, Tian M, Li M, Gu Z, Liao Q, Du Z*. Selective Degradation of Cucumber Mosaic Virus RNA3 by Nonsense-Mediated Decay Benefits Viral Early Infection. Mol Plant Pathol. 2025 Mar;26(3):e70070. doi: 10.1111/mpp.70070.
3. Qiao Z, Wang J, Huang K, Hu H, Gu Z, Liao Q, Du Z*. The non-template functions of helper virus RNAs create optimal replication conditions to enhance the proliferation of satellite RNAs. PLoS Pathog. 2024 Apr 17;20(4):e1012174. doi: 10.1371/journal.ppat.1012174.
4. Ahmed MR, Du Z*. Molecular Interaction of Nonsense-Mediated mRNA Decay with Viruses. Viruses. 2023 Mar 23;15(4):816. doi: 10.3390/v15040816.
5. Li M, Zhang X, Huang K, Du Z. Identification of Host Factors Interacting with a γ-Shaped RNA Element from a Plant Virus-Associated Satellite RNA. Viruses. 2023 Oct 1;15(10):2039. doi: 10.3390/v15102039.
6. Wang X, Lai J, Hu H, Yang J, Zang K, Zhao F, Zeng G, Liao Q, Gu Z*, Du Z*. Infection of Nigrospora nonsegmented RNA Virus 1 Has Important Biological Impacts on a Fungal Host. Viruses. 2022 Apr 12;14(4):795. doi: 10.3390/v14040795.
7. Gao Y, Yang J, Zhang X, Chen A, Gu Z, Du Z*. The Weak Small RNA-Binding Activity of the 2b Proteins of Subgroup II Cucumber Mosaic Virus Strains Is Insufficient for RNA Silencing Suppression. Front Microbiol. 2021 Oct 22;12:760937. doi: 10.3389/fmicb.2021.760937.
8. Ilyas M#, Du Z#, Simon AE*. Opium Poppy Mosaic Virus Has an Xrn-Resistant, Translated Subgenomic RNA and a BTE 3' CITE. J Virol. 2021 Apr 12;95(9):e02109-20. doi: 10.1128/JVI.02109-20. （# co-first author）
9. He L, Wang Q, Gu Z, Liao Q, Palukaitis P, Du Z*. A conserved RNA structure is essential for a satellite RNA-mediated inhibition of helper virus accumulation. Nucleic Acids Research, 2019, 47 (15): 8255-8271（IF：11.14）
10. Gao S, Lu J, Cheng X, Gu Z, Liao Q, Du Z*. Heterologous Replicase from Cucumoviruses can Replicate Viral RNAs, but is Defective in Transcribing Subgenomic RNA4A or Facilitating Viral Movement. Viruses. 2018 Oct 28;10(11):590. doi: 10.3390/v10110590.
11. Du Z, Alekhina O, Vassilenko K, Simon A; Concerted action of two 3΄ cap-independent translation enhancers increases the competitive strength of translated viral genomes, Nucleic Acids Research, 2017, 45 (16): 9558-9572（IF：11.56）
12. Du Z*, Chen A, Chen W, Westwood J, Baulcombe DC, Carr JP*. Using a Viral Vector to Reveal the Role of MicroRNA159 in Disease Symptom Induction by a Severe Strain of Cucumber mosaic virus. Plant Physiology, 2014, 164:1378-1388. (IF:7.39) Top Journal (IF：7.4)
13. Du Z*, Chen A, Chen W, Liao Q, Zhang H, Bao Y, Roossinck MJ, Carr JP*. Nuclear-cytoplasmic partitioning of the Cucumber mosaic virus 2b protein determines the balance between its roles as a virulence determinant and RNA silencing suppressor. Journal of Virology, 2014, 88(10): 5228-5241. (IF:4.68) Top Journal （IF：5.2）
14. Liao Q#, Tu Y#, Carr J.P*, Du Z*. An improved cucumber mosaic virus-based vector for efficient decoying of plant microRNAs. Scientific Reports 2015, 5:13178. (IF: 5.58)
15. Gao S#, Lu J#, Cheng X, Gu Z, Liao Q, Du Z*. Heterologous replicase from cucumoviruses can replicate viral RNAs, but is defective in transcribing subgenomic RNA4A or facilitating viral movement. Viruses. 2018,10 (11):590. doi: 10.3390/v10110590. (IF: 3.76)
16. 王妹妹, 陈文虎，廖乾生，杜志游*. MicroRNA 靶向病毒携带的microRNA 模拟靶序列对病毒的抑制作用. 生物化学与生物物理进展，2017, 44(2): 155-162. (SCI-E, 封面论文) (IF: 1.12)
17. Wang R, Du Z*, Liang Z*. The subcellular distribution of Cucumber mosaic virus LS2b protein, correlation between its nuclear localization and predicted nuclear localization signals. Physiological and Molecular Plant Pathology, 2017, 100:1-12.
18. Xu A, Zhao Z, Chen W, Liao Q, Chen J, Carr J, Du Z*. Self-interaction of the cucumber mosaic virus 2b protein plays a vital role in suppression of RNA silencing and induction of viral symptoms. Molecular Plant Pathology, 2013, 14(8), 803-812. (IF:3.89)
19. Du Z, Chen F, Zhao Z, Liao Q, Palukaitis P, Chen J. The 2b protein and the C-terminus of the 2a protein of cucumber mosaic virus subgroup I strains both play a role in viral RNA accumulation and induction of symptoms. Virology 2008, 380: 363-370. (IF:3.87)
20. Du Z-Y, Chen F-F, Liao Q-S, Zhang H-R, Chen Y-F, Chen J-S. The 2b ORFs encoded by subgroup IB strains of Cucumber mosaic virus induce different virulence on Nicotiana species. Journal of General Virology 2007, 88: 2596-2694. (IF: 3.37)
21. Du Z, Jin B, Liu W, Chen L, Chen J. Highly sensitive fluorescent-labeled probes and glass slide hybridization for the detection of plant RNA viruses and a viroid. Acta Biochimica et Biophysica Sinica 2007, 39(5): 326-334. (IF: 1.02)
22. Chen Y, Chen J, Zhang H, Tang X, Du Z*. Molecular evidence and sequence analysis of a natural reassortant between Cucumber mosaic virus subgroup IA and II strains. Virus Genes 2007, 35: 405-413. (IF: 2.43)
23. Du Z, Chen J, Hiruki C. Optimization and application of a multiplex RT-PCR for simultaneous detection of five potato viruses using 18S rRNA as an internal control. Plant Disease 2006, 90(2): 185-189. (IF: 2.91)
24. Du Z-Y, Chen J-S. Identification and grouping study of Potato virus X Hunan isolate. Virologica Sinica 2003, 18(2):119-123. (In Chinese)
---共同作者论文
1. Tungadi T, Watt LG, Groen SC, Murphy AM, Du Z, Pate AE, Westwood JH, Fennell TG, Powell G, Carr JP. Infection of Arabidopsis by cucumber mosaic virus triggers jasmonate-dependent resistance to aphids that relies partly on the pattern-triggered immunity factor BAK1. Mol Plant Pathol. 2021 Sep;22(9):1082-1091. doi: 10.1111/mpp.13098. Epub 2021 Jun 22. PMID: 34156752; PMCID: PMC8358999.
2. Du K, Jiang T, Chen H, Murphy AM, Carr JP, Du Z, Li X, Fan Z, Zhou T. Viral Perturbation of Alternative Splicing of a Host Transcript Benefits Infection. Plant Physiol. 2020 Nov;184(3):1514-1531. doi: 10.1104/pp.20.00903. Epub 2020 Sep 21. PMID: 32958561; PMCID: PMC7608148.
3. Chang F, Gu Z, Zhu P, Lu R, Du Z, Liao Q. Reduction of RNA-dependent RNA polymerase 6 in Nicotiana benthamiana enhances gene silencing induced by Foxtail mosaic virus. Acta Biochim Biophys Sin (Shanghai). 2017 Jul 1;49(7):652-654. doi: 10.1093/abbs/gmx050. PMID: 28541405.
4. Wang R, Du Z, Bai Z, Liang Z. The interaction between endogenous 30S ribosomal subunit protein S11 and Cucumber mosaic virus LS2b protein affects viral replication, infection and gene silencing suppressor activity. PLoS One. 2017 Aug 14;12(8):e0182459. doi: 10.1371/journal.pone.0182459. PMID: 28806733; PMCID: PMC5555695.
5. Li C, Du Z, Chen J. Structural and functional elucidation of AGO proteins. Chinese Journal of Biochemistry and Molecular Biology, 2009, 25(11): 969-976.
6. Li L, Tian Q, Du Z, Duns GJ, Chen J. A novel double-stranded RNA virus detected in Primula malacoides is a a plant-isolated partitivirus closely related to partitivirus infecting fungal species. Archive Virology, 2009, 164(4): 565-572.
7. Liao Q-S, Du Z-Y, Zhang H-R, Wu P, Zhu L-P, Chen J-S. Construction of infectious clone for cucumber mosaic virus pepper isolate. Scientia Agricultura Sinica 2008, 41(7): 1975-1982. (In Chinese)
8. Chen Y, Chen J, Zhang H, Tang X, Du Z. Molecular evidence and sequence analysis of a natural reassortant between Cucumber mosaic virus subgroup IA and II strains. Virus Genes 2007, 35: 405-413.
9. Liao Q, Zhu L, Du Z, Zeng R, Peng J, Chen J. Satellite RNA-mediated reduction of cucumber mosaic virus genomic RNAs accumulation in Nicotiana tabacum. Acta Biochimica et Biophysica Sinica 2007, 39(3):217-23.
10. Chen F-F, Du Z-Y, Liu X, Xie L, Chen J-S. Effect of Cucumber mosaic virus-encoded 2b protein on Photosynthesis and chloroplast structure of the host plant. Progress in Biochemistry and Biophysics 2007, 34(8): 889-896. (In Chinese)
11. Liao Q-S, Du Z-Y, Zhang H-R, Zhu L-P., Wu P, Chen J-S. Necrosis pathotype induced on Nicotiana glutinosa by infection of CMV-Cb7 related to RNA2. Progress in Biochemistry and Biophysics 2007, 34(8): 824-829. (In Chinese)
12. Lang Q-L, Chen J-S, Fu T-Z, Du Z-Y. Occurrence and molecular detection of Potato virus S (PVS) in Zhejiang province. Acta Phytopathology Sinica 2007, 32(2): 217-220. (In Chinese)
13. Feng J, Chen S, Tang X, Ding X, Du Z, Chen J. Quantitative determination of cucumber mosaic virus genome RNAs in virions by real-time reverse transcription-polymerase chain reaction. Acta Biochimica et Biophysica Sinica 2006, 38(10):669-676.
14. Gu Y, Du Z-Y, Lang Q-L, Chen J-S, He N-Y. Detection of Potato Spindle Tuber Viroid Using RNA Hybridized Chips. Chemical Research in Chinese Universities 2006, 27(11):2106-2109. (In Chinese)
15. Dong X-J*, Du Z-Y, Chen Z. Decolorization of Direct Black 22 by Aspergillus ficuum. Journal of Environmental Sciences 2001, 12(4): 472-475.
---授权发明专利
1. CMV卫星RNA生防型突变体的构建方法及其在番茄上的应用，杜志游，赵丹青，黄开云，廖乾生，专利号ZL 2025 1 1163735.0，2026.01授权.
2. 同时表达两个外源蛋白载体TRVe2的构建方法与应用，廖乾生、常发光、杜志游、刘小红、林福呈，专利号：ZL201810261990.2，2021年09月授权.
3. 基于TRV的CRISPR/Cas12a基因组编辑载体的构建与应用，廖乾生、郭歌、赖家良、杜志游，专利号：ZL202210682700.8 ， 2024年12月授权.
4. 一种可用于防治黄瓜花叶病毒的RNA分子及作物抗病毒制剂，金伟波、陈强、吴方丽、杜志游，专利号：ZL202210376297.6，2023年12月授权.
5. 利用烟草脆裂病毒同时表达两种外源蛋白的载体构建法，廖乾生、郭歌、常发光、赖家良、杜志游，专利号：ZL202110836772.9，2023年05月授权.
（六）学生培养情况
1. 国家奖学金获得者：陈爱中（2012级），赵丹青（2023级）
2. 省优秀硕士学位论文：何露（2016级），乔子木（2021级）
3. 省优秀毕业生：赵丹青（2023级）
