讲座名称： Cell biocontrol technology:

Physical stimulation can easily control cell behavior

报告人：Jong-Chul Park, 博士，教授，韩国延世大学医学院医学工程主席，韩国延世大学医疗技术&质量评价中心主任，韩国生物材料协会副主席

时间： 2016年6月6日 15:00-16:00

地点： 浙江理工大学18号楼218室

学术背景：

1991-1995 日本东京农工大学生物技术学博士学位

1988-1990 韩国首尔国立大学公共卫生学硕士学位

1983-1987 韩国延世大学生物化学学士学位

研究领域：

生物相容性，细胞迁移，生物活性物质，组织工程和再生医学

报告内容：

Background

This presentation deals with the cell biocontrol technology using physical stimulations. Chemical stimulation could affect the cell behaviors too; however, it is not easy to remove the influence of the first treated chemical when the second chemical is acting on the cells which are already affected by the first chemical to change the cell behavior. Therefore we prefer to use physical stimulation to control the cell behavior. In this talk, I will present few physical stimulation methods for controlling cell behavior; photosensitizer incorporated polymer with light source, fluid shear stress and electric current.

Methodology/Principal Findings

In photosensitizer incorporated polymer part, we use the reactive oxygen species (ROS) regulation system which consists of hematoporphyrin (Hp) contained materials and green light emitting diode (LED) stimulator. The proliferation of human vascular endothelial cells (HUVEC) were significantly increased when the green LED was treated with 470µW/cm2 for 30 min to the cell seeded Hp-contained poly urethane (PU) film (1µg/cm2). However, increased light power (3mW/cm2 for 30 min) induced the decrease of proliferation in same Hp-PU film. We also found that the viability of human bladder cancer cell (T-24) was significantly decreased in Hp-PU film (10µg/cm2) with light source (5mW/cm2 for 30 min). Cell detachment and transfer technology was studied too. Neonatal human dermal fibroblast (nHDF) and human mesenchymal stem cells (hMSC) were transferred from Hp contained polyketone film to the fibrin gel when the light source was treated. In control of cell migration part, two physical stimulation methods; mechanotaxis and electrotaxis were studied. 8 dyne/cm2 of fluid shear stress induced the directional migration of HUVECs. The fluid shear stress also enhanced the infiltration of hMSCs into the poly(lactic-co-glycolic acid) scaffold. In electrotaxis study, nHDFs showed directional migration towards the cathode in direct current (dc) EFs (1 V/cm) and they moved in the opposite direction when the polarity of the dc EF was reversed. Reorganization of the actin cytoskeleton and polarization of the Golgi apparatus were evaluated by immunostaining, which showed that the actin cytoskeleton elongated towards the cathode and the Golgi apparatus polarized in the direction of the dc EF. Adipose derived stem cells (ADSC) and osteogenic differentiated ADSC were migrated toward anode however the migration speed of osteogenic differentiated ADSC was significantly decreased. We also identified the distinguishment of ADSC vs. osteogenic differentiated ADSC using the electrotaxis migration data analysis method.

Conclusion and Significance

In conclusion, we studied about cell biocontrol technology with the physical stimulations; light source with Hp, fluid shear stress and electric current. With these stimulations, we controlled the cell proliferation, cancer therapy, cell transfer, cell infiltration and migration. As a result, we suggest that the physical stimulation can be appropriate method for control of cell behaviors.