潘建义 性 别： 男 出生年月：1977年1月 职 称： 副教授 所在学科组：生物化学与分子生物学 办 公 室：6号楼305室 电 话：0571-8684 3747 电子邮件：jianyi.pan@zstu.edu.cn

主要学习工作经历

2010.10-至 今 浙江理工大学副教授、硕士生导师

2014.09-2015.09 美国芝加哥大学 国家公派访问学者

2008.09-2010.09 浙江理工大学讲师

2005.09-2008.06 厦门大学博士研究生

主要学术及社会兼职

中国生物化学与分子生物学学会会员

中国细胞生物学会会员

Journal of Bioinformatics and Proteomics (SM)期刊编委会成员

J Proteome Res, Sci Rep, Trends Microbiol, BMC Microbiol, PloS One等十多种期刊审稿

主要研究方向

• 病原菌与宿主细胞互作的蛋白质翻译后修饰及信号转导研究

• 外膜蛋白在病原菌致病中的作用及分子机制研究

• 重要病原菌外膜蛋白的疫苗靶位的筛选和鉴定

在研科研及教学项目

• 国家自然科学基金：副溶血弧菌T3SS2效应蛋白VPA1324操控宿主cdG免疫信号通路的肠毒性机制，项目主持人

• 浙江省自然科学基金面上项目：铜绿假单胞菌PpkA磷酸化网络及其对耐药的调控机制研究，项目主持人

• 国家自然科学基金：副溶血弧菌VP0057介导蛋白质磷酸化修饰调控毒力的分子机制, 参与（排名2/7）

• 国家自然科学基金：金属富勒烯的质谱研究，参与（排名2/7）

2010年以来主要论文（* 通讯作者）

• Jianyi Pan*, Zhenzhong Zha, Pengfei Zhang, Ran Chen, Chen Ye, Ting Ye. Serine/threonine protein kinase PpkA contributes to the adaptation and virulence in Pseudomonas aeruginosa. Microbial Pathogenesis, 2017, 113: 5-10. (Impact Factor: 2.009)

• 李楚楚，李伟燕，潘建义*. 副溶血弧菌Ⅲ型分泌系统(T3SS)效应蛋白及其对宿主细胞的操控. 中国生物化学与分子生物学报，2017, 33(3): 247-251.

• Zha Z, Li C, Li W, Ye Z, Pan J*. LptD is a promising vaccine antigen and potential immunotherapeutic target for protection against Vibrio species infection. Scientific Reports. 2016, 6: 38577. (Impact Factor: 5.228)

• Li W, Wen L, Li C, Chen R, Ye Z, Zhao J, Pan J*. Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H. Microbial Pathogenesis. 2016, 98:57-62. (Impact Factor: 1.888)

• Pan J*, Li C, Ye Z. Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins. Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases. Chapter 34, Springer New York, 2016.

• Pan J*, Chen R, Li C, Li W, Ye Z. Global Analysis of Protein Lysine Succinylation Profiles and Their Overlap with Lysine Acetylation in the Marine Bacterium Vibrio parahemolyticus. Journal of Proteome Research. 2015, 14(10):4309-18. (Impact Factor: 4.353)

• Wang KZ, Weng LY, Ye ZC, Wu HG, Pan JY*. Hemocyanin-derived phenoloxidase activity is dependent on dodecameric structure in shrimp Litopenaeus vannamei. Archives of Biological Sciences. 2015, 67(1):19−23.

• Pan J*, Ye Z, Cheng Z, Peng X, Wen L, Zhao F. Systematic Analysis of the Lysine Acetylome in Vibrio parahemolyticus. Journal of Proteome Research. 2014, 13(7):3294-3302. (Impact Factor: 5.001)

• Li C, Ye Z, Wen L, Chen R, Tian L, Zhao F, Pan J*. Identification of a novel vaccine candidate by immunogenic screening of Vibrio parahaemolyticus outer membrane proteins. Vaccine. 2014, 32(46): 6115–6121. (Impact Factor: 3.624)

• 潘建义*, 王长一, 叶智鸧, 陈冉, 赵辅昆. 巨噬细胞应激的铜绿假单胞菌磷酸化蛋白质组分析. 中华微生物学和免疫学杂志, 2014, 34(2): 91-95.

• Wu XB, Tian LH, Zou HJ, Wang CY, Tang CH, Yu ZQ, Zhao FK, Pan JY*. Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis. Research in Microbiology. 2013, 164(8): 848-855. (Impact Factor: 2.826)

• 田丽花, 叶智鸧, 温良优, 潘建义*, 赵辅昆. 副溶血弧菌免疫原性蛋白的鉴定及克隆表达. 浙江理工大学学报, 2013, 30(6): 881-886.

• Li H, Pan JY, Liu XJ, Gao JX, Wu HK, Wang C, Peng XX. Alterations of protein complexes and pathways in genetic information flow and response to stimulus contribute to Escherichia coli resistance to balofloxacin. Molecular BioSystems. 2012, 8(9):2303-2311.

• Zhao J, Wen G, Ding M, Pan JY, Yu ML, Zhao F, Weng XL, Du JL. Comparative proteomic analysis of colon cancer cell HCT-15 in response to all-trans retinoic acid treatment. Protein and Peptide Letters. 2012, 19(12):1272-1280.

• Pan JY, Wu H, Liu X, Li PP, Li H, Wang SY, Peng XX. Complexome of Escherichia coli cytosolic proteins under normal native conditions. Molecular BioSystems. 2011, 7(9):2651-2663.

• 吴贤斌, 邹海杰, 田丽花, 潘建义*, 赵辅昆. 大肠杆菌外膜蛋白ompW基因敲除菌的构建及其对两种抗生素的敏感性. 微生物学报, 2012, 52(8):1021- 1026.

• 邹海杰, 吴贤斌, 潘建义*, 赵辅昆. 大肠杆菌外膜蛋白OmpW的克隆及在外膜上高表达. 中国生物工程杂志, 2011, 31(12):46-50.

• 潘建义*, 吴海港, 王浩波. 对虾血蓝蛋白多聚结构与凝集活性. 中国病原生物学杂志, 2011, 6(1): 26-28.

• Pan JY, Li H, Ma Y, Chen P, Zhao P, Wang SY, Peng XX*. Complexome of Escherichia coli envelope proteins under normal physiological conditions. Journal of Proteome Research. 2010, 9(7):3730-3740.

• PAN Jian-Yi *, YU Zhi-Qiang. Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide from the Chinese bird spider (Haplopelma hainanum). Zoological Research, 2010, 12; 31(6): 570−574.

• Pan JY*, Chen P, Wang SY. Modification of energy metabolism-related proteins is responsible for ceftriaxone-resistance. Chin J Biochem Mol Biol, 2010, 26(2): 140-149.

重要会议论文(摘要)

• 李楚楚，查振中，李伟燕，潘建义*.海洋弧菌外膜蛋白LptD: 潜在的疫苗靶位及药物靶点. 第十届全国海洋生物技术与创新药物暨海洋生化学术会议, 2016年8月20-24日，锦州.

• 王长一, 潘建义*, 赵辅昆. 巨噬细胞应激的铜绿假单胞菌磷酸化蛋白质组分析. 第四届全国“跨学科蛋白质研究”学术研讨会（蛋白质与人类健康）， 2013年10月12-14日，合肥.

• Jian-yi Pan, San-Ying Wang, Xuanxian Peng. Cytoplasmic protein complexome involved in ceftriaxone resistance in Escherichia coli. The HUPO2012 11th Annual World Congress conference, September 9-13 in Boston, MA in the United States.

• Jian-Yi Pan, Hui Li, Sanying Wang, Xuanxian Peng. Protein Complexome of Escherichia Coli Cell Envelope Under Proximately Physiological Conditions. 2010 AIChE Annual Meeting: Salt Lake City, UT, USA.

• Xian-bing Wu, Li-hua Tian, Hai-jie Zou, Chang-yi Wang, Zhi-qiang Yu, Chen-hong Tang, Fu-kun Zhao, Jian-yi Pan*. Vaccine candidate outer membrane protein OmpW of Escherichia coli is required for resistance to host innate immunity. 2012年全国生物化学与分子生物学学术大会, 8月24-26, 成都.

发明专利

• 专利号：201210275708.9；专利授权日：2015年7月22日；申请人：浙江理工大学；发明名称：一种以大肠杆菌外膜孔蛋白ompW为靶点筛选药物的方法；发明人：潘建义，吴贤斌，邹海杰.

• 专利申请号：201410475962.2；专利申请日：2014年09月17日；申请人：浙江理工大学；发明创造名称：一种副溶血弧菌外膜蛋白LptD的制备方法及其应用；发明人：潘建义，李楚楚，叶智鸧，温良优，陈冉，田丽花.

已结题科研及教学项目

• 国家自然科学基金：铁离子调控大肠埃希菌O157:H7外膜孔蛋白OmpW 抗先天免疫的分子机制，项目主持人

• 浙江省自然科学基金项目：沙瑞度坦抑制人粒性白血病细胞增殖的靶点蛋白筛选及其功能研究，参与（排名2/5）

• 浙江省高校生物学重中之重（一级）学科优秀青年基金：大肠埃希菌外膜蛋白OmpW抗氧化应激的分子机制及铁离子的调控作用，项目主持人

• 浙江省自然科学基金：铁离子介导细菌外膜OmpW蛋白复合物抗先天性免疫研究，项目主持人

• 浙江省科技厅公益技术项目：铁离子介导病原菌毒力蛋白OmpW抗先天性免疫，项目主持人

• 浙江省教育厅科技项目：凡纳对虾血蓝蛋白的多聚体结构与免疫活性关系研究，项目主持人

• 浙江理工大学科研项目：铁在细菌感染发病机制中介导菌外膜蛋白OmpW与吞噬细胞的相互作用研究，项目主持人

• 浙江理工大学教改项目：《生物化学》精品网络课程建设项目，主持人

JIANYI PAN

Address: Room 305, Building 6, Zhejiang Sci-Tech University, Xiasha Higher Education Zone, Hangzhou 310018, Zhejiang, China.

Tel: +86-571-8684 3747

E-mail: jianyi.pan@zstu.edu.cn

EDUCATIONAL & WORKING EXPERIENCE

• 9/2008 to present Associate Professor at College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China

• 9/2014-9/2015 Research scholar at The University of Chicago, Chicago, USA

• 9/2005-6/2008 Ph.D. in Biochemistry (Specialize in Immune Proteomics), Xiamen University, Xiamen, China

• 9/2002-7/2005 Teaching Assistant and Lecturer in School of Biotechnology and Food Engineering, Heifei University of Technology, Hefei, China.

RESEARCH AREA

• Identification and characterization of post-translation modification (PTM) of bacterial proteins.

• Identification and functional analysis of the outer membrane proteins and their protein complexes in gram-negative bacteria.

• Screening and identification of vaccine candidate from outer membrane proteins of pathogenic bacteria through high-throughput immunoproteomics.

GRANTS SPONSORED BY GOVERNMENTS (selected)

• Natural Science Foundation of Zhejiang Province (No. LY15C010004)

Regulatory mechanisms for drug resistance by STPK phosphorylation network in Pseudomonas aeruginosa.

• National Natural Science Foundation of China (No. 31200110)

Molecular mechanism of OmpW resistance to host innate immune regulated by iron in Escherichia coli O157:H7.

• Natural Science Foundation of Zhejiang Province (No. Y2090396)

Iron-mediated bacterial outer membrane protein OmpW complex resistance to host innate immune.

• Science & Technology Project from Educational Commission of Zhejiang Province (No. Y200805989)

Research on relationship between oligomeric structure and immune activity of Hemocyanin from Penaeus vannamei.

PROFESSIONAL QUALIFICATIONS

• 2007-present Member of Chinese Society for Cell Biology

• 2010-present Member of Chinese Society of Biochemistry and Molecular Biology

• Editor Board Member of Journal of Bioinformatics and Proteomics

• Reviewer of J Proteome Res, Sci Rep, PloS One, BMC Microbiol, et al.

TRACKABLE WORK RECORDS

Publications (2010 to present) (* Corresponding author)

• Zha Z, Li C, Li W, Ye Z, Pan J*. LptD is a promising vaccine antigen and potential immunotherapeutic target for protection against Vibrio species infection. Sci Rep. 2016, 6: 38577.

• Li W, Wen L, Li C, Chen R, Ye Z, Zhao J, Pan J*. Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H. Microb Pathog. 2016, 98:57-62.

• Pan J*, Li C, Ye Z. Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins. Vaccine Design: Methods and Protocols, Chapter 34, Springer New York, 2016.

• Pan J*, Chen R, Li C, Li W, Ye Z. Global Analysis of Protein Lysine Succinylation Profiles and Their Overlap with Lysine Acetylation in the Marine Bacterium Vibrio parahemolyticus. J Proteome Res. 2015, 14(10):4309-18.

• Wang KZ, Weng LY, Ye ZC, Wu HG, Pan JY*. Hemocyanin-derived phenoloxidase activity is dependent on dodecameric structure in shrimp Litopenaeus vannamei. Arch Biol Sci. 2015, 67(1):19−23.

• Pan J*, Ye Z, Cheng Z, Peng X, Wen L, Zhao F. Systematic Analysis of the Lysine Acetylome in Vibrio parahemolyticus. J Proteome Res. 2014, 13(7):3294-3302.

• Pan J*, Wang C, Ye Z, Chen R, Zhao F. Phosphoproteomic analysis of Pseudomonas aeruginosa strains in response to stress induced by macrophages. Chin J Microbiol Immuno. 2014, 34(2): 91-95.

• Wu XB, Tian LH, Zou HJ, Wang CY, Tang CH, Yu ZQ, Zhao FK, Pan JY*. Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis. Res Microbiol. 2013, 164(8): 848-855.

• Pan JY, Li H, Ma Y, Chen P, Zhao P, Wang SY, Peng XX. Complexome of Escherichia coli envelope proteins under normal physiological conditions. J Proteome Res. 2010, 9(7):3730-3740.

• Li H, Pan JY, Liu XJ, Gao JX, Wu HK, Wang C, Peng XX. Alterations of protein complexes and pathways in genetic information flow and response to stimulus contribute to Escherichia coli resistance to balofloxacin. Mol Biosyst. 2012, 8(9):2303-2311.

• Pan JY, Wu H, Liu X, Li PP, Li H, Wang SY, Peng XX. Complexome of Escherichia coli cytosolic proteins under normal native conditions. Mol Biosyst, 2011, 7(9):2651-2663.

• Zhao J, Wen G, Ding M, Pan JY, Yu ML, Zhao F, Weng XL, Du JL. Comparative proteomic analysis of colon cancer cell HCT-15 in response to all-trans retinoic acid treatment. Protein Pept Lett. 2012, 19(12):1272-1280.

• Tian LH, Ye ZC, Wen LY, Pan JY*, Zhao FK. Identification and Expression of the Immunogenic Protein of Vibrio Parahaemolyticus. Journal of Zhejiang Sci-Tech University, 2013, 30(6): 881-886.

• Wu XB, Zou HJ, Tian LH, Pan JY*, Zhao FK. Construction of ompW knock-out mutants of Escherichia coli. Acta Microbiol Sin, 2012, 52(8):1021- 1026.

• Pan JY*, Wu HG, Wang HB. Differences in the agglutination activity of two oligomers of hemocyanin from Litopenaeus vannamei. J of Pathogen Biol, 2011, 6(1): 26-28.

• Zou HJ, Wu XB, Pan JY*, Zhao FK. Cloning and expression of ompW location in outer membrane of Escherichia coli. Chin Biotechnol, 2011, 31(12):46-50.

• Pan JY*, Yu ZQ. Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide from the Chinese bird spider (Haplopelma hainanum). Zoological Research, 2010, 12; 31(6): 570-574.

• Pan JY*, Chen P, Wang SY. Modification of energy metabolism-related proteins is responsible for ceftriaxone-resistance. Chin J Biochem Mol Biol, 2010, 26(2): 140-149.

• Chen W, Sun J, Du YG, Li SG, Pan JY, Yuan ZY, Zhao FK. Studies on immobilization of exoinulinase from Kluyveromyces cicerisporus Y-179. Ind Microbiol, 2010(6): 13-18.

Conference Presentations (selected)

• Jian-yi Pan, San-Ying Wang, Xuanxian Peng. Cytoplasmic protein complexome involved in ceftriaxone resistance in Escherichia coli. The HUPO2012 11th Annual World Congress conference, September 9-13, 2012, Boston, USA.

• Jian-Yi Pan, Hui Li, Sanying Wang, Xuanxian Peng. Protein Complexome of Escherichia Coli Cell Envelope Under Proximately Physiological Conditions. 2010 AIChE Annual Meeting: Salt Lake City, UT, USA.

• Xian-bing Wu, Li-hua Tian, Hai-jie Zou, Chang-yi Wang, Zhi-qiang Yu, Chen-hong Tang, Fu-kun Zhao, Jian-yi Pan*. Vaccine candidate outer membrane protein OmpW of Escherichia coli is required for resistance to host innate immunity. 2012 Na,tional Congress of Chinese Association of Biochemistry and Molecular Biology, August 24-26, Chengdu, China.

• Jian-Yi Pan*, Hai-Jie Zou, Xian-Bin Wu. A novel mechanism of iron-mediated infectious disease: bacterial virulence protein complex resistance to host innate immunity. The 10th Congress conference of Chinese Association of Biochemistry and Molecular Biology, August 23-25, Nanjing, China.