主要学习工作经历

1. 2008.09~至今 浙江理工大学 生命科学学院 副研究员

2. 2006.04~2008.08 浙江理工大学 生命科学学院 助理研究员

3. 1999.09~2006.03 浙江大学 生物化学与分子生物学专业 博士

4.1995.09~1999.06 华中农业大学 畜牧兽医学院兽医专业 本科

主要学术及社会兼职

1. 中国生物化学与分子生物学学会会员

主要研究方向

1. 家蚕生物反应器

2. 杆状病毒分子生物学

获奖与荣誉

1.

2.

3.

科研教学项目

1. 浙江省自然科学基金项目, 复制缺陷型家蚕BmNPV高通量载体表达系统的构建及应用研究, Y309028, 2010.01~2011.12。

2. 浙江省自然科学基金项目, 家蚕生物反应器高效表达蛇毒金属蛋白酶Agkistin及其抗血栓等功能的研究 Y305171, 2006.01~2007.12。

3. 国家863项目，家蚕生物反应器蛋白质药物规模化制备技术平台2007AA021703， 2007.07~2010.11。

专著论文

1. 费伟强, 陈琴, 陈倩, 梅文枫, 边腾飞, 吕正兵, 于威, 陈健*, 张耀洲, 吴祥甫. 复制缺陷型BmNPV载体的构建及初步应用. 蚕业科学, 2013, 39（3）：0501-0508.

2. 陈和, 陈琴, 梅文枫, 钱月忠, 聂作明, 于威, 陈健*, 张耀洲. 一种红色荧光蛋白基因稳定转化的家蚕细胞株构建及应用. 蚕业科学, 2013, 39（3）：0494-0500.

3. 陈琴, 杨丹, 聂作明, 陈健, 张耀洲. 家蚕脂蛋白基因BmLp-c21的表达及亚细胞定位. 蚕业科学, 2012, 39（3）：0434-0441.

4. Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Wang L, Indran IR, Li J, Qian L, Lv Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Mar Drugs. 2013, 11(5):1492-1505.

5. Yu W, Du CY, Quan YP, Nie ZM, Chen J, Lv ZB, Zhang YZ. Characterization of late gene expression factor LEF-10 from Bombyx mori nucleopolyhedrovirus. Virus Res. 2013, 175(1): 45-51.

6. Zhou F, Gao Z, Lv Z, Chen J, Hong Y, Yu W, Wang D, Jiang C, Wu X, Zhang Y, Nie Z. Construction of the ie1-Bacmid Expression System and Its Use to Express EGFP and BmAGO2 in BmN Cells. Appl Biochem Biotechnol. 2013 Apr; 169(8): 2237-2247.

7. Yang L, Lu X, Liu Y, Lv Z, Chen J, Yu W, Zhang Y, Nie Z. Expression analysis of miRNAs in BmN cells. Gene. 2012 Sep 1; 505(2): 240-245.

8. Yu W, Li J, Wang M, Quan Y, Chen J, Nie Z, Lv Z, Zhang Y. The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virol J. 2012 May 6; 9:90.

9. Quan Y, Liu G, Yu W, Nie Z, Chen J, Lv Z, Zhang Y. Expression, Purification, and Characterization of Ras Protein (BmRas1) from Bombyx mori. Comp Funct Genomics. 2012; 2012:747539.

10. Lv Z, Zhang X, Liu L, Chen J, Nie Z, Sheng Q, Zhang W, Jiang C, Yu W, Wang D, Wu X, Zhang S, Li J, Zhang Y. Characterization of a gene encoding prohibitin in silkworm, Bombyx mori. Gene. 2012 Jul 10; 502(2):118-124.

11. Chen J, Gai Q, Lv Z, Chen J, Nie Z, Wu X, Zhang Y. All-trans retinoic acid affects subcellular localization of a novel BmNIF3l protein: functional deduce and tissue distribution of NIF3l gene from silkworm (Bombyx mori). Arch Insect Biochem Physiol. 2010 Aug; 74(4): 217-231.

12. Lan H, Nie Z, Liu Y, Lv Z, Liu Y, Quan Y, Chen J, Zhen Q, Chen Q, Wang D, Sheng Q, Yu W, Chen J, Wu X, Zhang Y. In vivo bioassay of recombinant human growth hormone synthesized in B. mori pupae. J Biomed Biotechnol. 2010; 2010:306462.

13. Zeng F, Xie H, Nie Z, Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang Y. Characterization of the gene BmEm4, a homologue of Drosophila E(spl)m4, from the silkworm, Bombyx mori. Comp Funct Genomics. 2009: 947490.

14. Shen Y, Zhang T, Chen J, Lv Z, Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q, Wu X, Zhang Y. Molecular characterization and tissue localization of an F-box only protein from silkworm, Bombyx mori. Comp Funct Genomics. 2009: 416040.

15. Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics. 2009 Nov; 9(4):447-454.

16. Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, Chen G, Zheng Q, Jin L, Wu X, Chen J, Zhang Y. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori. PLoS One. 2008; 3(12):e3933.

17. He PA, Nie Z, Chen J, Chen J, Lv Z, Sheng Q, Zhou S, Gao X, Kong L, Wu X, Jin Y, Zhang Y. Identification and characteristics of microRNAs from Bombyx mori. BMC Genomics. 2008 May 28; 9:248.

18. Lingyin Kong, Zhengbing Lv, Jian Chen, Zuoming Nie, Dan Wang, Hongdan Shen, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori). BBA-General subject, 2007 Dec;1770(12):1598-1604.

19. Zhang YZ, Chen J, Nie ZM, Lu ZB, Wang D, Jiang CY, He PA, Liu LL, Lou YL, Song L, Wu XF. Expression of open reading frames in silkworm pupal cDNA library. Appl Biochem Biotechnol, 2007 Mar; 136(3):327-343.

20. Wang X, Chen J, Lv Z, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression and Functional Analysis of CRABP from Silkworm Pupae (Bombyx mori). J Cell Biochem, 2007 Nov 1; 102(4): 970-997.

21. Chen J, Nie ZM, Lv ZB, Zhu CG, Xu CZ, Jin YF, Wu XF, Zhang YZ. Large-Scale Purification of hGM-CSF Expressed in Bombyx mori Pupae. Appl Biochem Biotechnol. 2007 Apr; 141(1):149-160.

22. Chen J, Liu T, Zhu CG, Jin YF, Zhang YZ. Genetic variation of Chinese PRRSV Strains based on ORF5 sequence. Biochem Genet. 2006 Oct, 44(9-10): 425-435.

23. Chen J, Wu XF, Zhang YZ. Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor. J Biotechnol. 2006 May 17; 123(2): 236-247.

发明专利

1. 可线性化穿梭载体BmBacmid的构建方法：申请号：CN201210037290，已授权。

2. 稳定转化家蚕细胞株滴定BmNPV法及报告基因构建法：申请号：201310070843.4，已公开。